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The clinical trials allowed for dose adjustment based on tolerability, and your protocol should too

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Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2

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Gut microbiota-dependent trimethylamine N-oxide (TMAO) pathway contributes to both development of renal insufficiency and mortality risk in chronic kidney disease